Preparation of 2 3-dihydro-1h-1 4-benzodiazepines 4-oxides

ABSTRACT

2,3-DIHYDRO-1H-1,4-BENZODIAZEPINE 4-OXIDES ARE PREPARED FROM CORRESPONDINGLY SUBSTITUTED 2-(2-A-ETHYL LOWER ALKYLAMINO) BENZOPHEONE, WHERE A IS A LEAVING GROUP, BY TREATMENT OF THE LATTER COMPOUNDS WITH HYDROXYLAMINE HYDROCHLORIDE. ALTERNATIVELY, THE AFORESAID COMPOUNDS MAY BE OBTAINED BY CYCLIZTION OF 2-(2-AETHYL LOWER ALKYLAMINO) BENZOPHENONE OXIMES. THE 2,3DIHYDRO-1H-1,4-BENZODIAZEPINE 4-OXIDES ARE USEFUL AS INTERMEDIATES IN THE PREPARATION OF PHARAMACOLOGICALLY ACITVE 2,3-DIHYDRO-1H-1,4-BENZODIAZEPINES BY DEOXYGENATION OF THE FORMER COMPOUNDS.

United States Patent 3,464,011 PREPARATION OF 2,3-DIHYDRO-1H-1,4-

BENZODIAZEPINES 4-0XIDES Giles Allan Archer, Essex Fells, and Leo Henryk Stembach, Upper Montclair, N.J., assignors to Hotfmann- La Roche Inc., Nutley, NJ.

No Drawing. Continuation-impart of application Ser. No. 763,004, Sept. 26, 1968. This application Apr. 25, 1969, Ser. No. 819,410

Int. Cl. C07d 53/06 US. Cl. 260-239 BD Claims ABSTRACT OF THE DISCLOSURE RELATED APPLICATIONS The present application is a continuation-in-part of US. patent application Ser. No. 763,004, filed Sept. 26, 1968, now US, Pat. 3,583,978.

BRIEF DESCRIPTION OF THE INVENTION The present invention relates to the preparation of compounds of the following formula wherein R and R each are selected from the group consisting of hydrogen, halogen, trifiuoromethyl, nitro, amino, lower alkanoylamino, lower alkylthio, lower alkyl sulfonyl, lower alkylsulfinyl, lower alkoxy, hydroxy, lower alkyl, cyano, carboxy, carbo-lower alkoxy, carbamyl, and di-lower alkylamine; and R is selected from the group consisting of hydrogen, lower alkyl, lower alkenyl and lower alkanoyl, by, in one embodiment, a process wherein a compound of the following formula 3,646,011 Patented Feb. 29, 1972 wherein R R and R are as above and A is a leaving group, is reacted with hydroxylamine.

As used in this disclosure, the term lower alkyl comprehends both straight and branched chain carbonhydrogen radicals having from 1 to 7, preferably from 1 to 4 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl and the like. The term lower alkenyl includes straight and branched chain carbon-hydrogen radicals having from 1-7, preferably from 1-4 carbon atoms, wherein at least one carbon to carbon bond is unsaturated such as allyl and the like. The term lower alkanoyl includes the acyl residue of lower alkanoic acids, for example, acetyl, propionyl, and the like. The term halogen encompasses all four halogens, e.g., fluorine, chlorine, bromine and iodine.

In a preferred embodiment of the present invention, compounds of Formula I above are prepared wherein R and R are selected from the group consisting of hydrogen, halogen, trifluoromethyl and nitro and R is lower alkyl. In a more preferred embodiment R is halogen, most preferably chlorine and R is hydrogen. In the most preferred embodiment, R is methyl, R is substituted at the 7-position of the benzodiazepine ring and R when other than hydrogen is preferably substituted at the 2-position.

The process for preparing compounds of the Formula I from corersponding compounds of Formula II above by means of a reaction with hydroxylamine or a salt thereof, e.g., hydroxylamine hydrochloride, may be conducted in various ways. In one preferred embodiment, the reaction between compounds of Formula II and hydroxylamine is conducted in a suitable solvent such as aqueous lower alkanol in the presence of a base such as a carbonate, e.g., potassium or sodium carbonate, or an alkali or alkaline earth hydroxide, etc.

The reaction may also be carried out in excess hydroxylamine which serves both as solvent and as base in the reaction. Alternatively, the hydroxylamine may be admixed with an organic base, such as pyridine or triethylamine which serve as solvents and also as added bases. A preferred embodiment of the present invention utilizes as a reaction mixture hydroxylamine hydrochloride in ethanol to which has been added potassium carbonate in water.

The aforesaid reaction for this preparation of compounds of Formula I is generally carried out in a tem perature range of 0 to the reflux temperature of the solvent medium. In preferred embodiments the aforesaid reaction is conducted at a temperature in the range of from room temperature to the reflux temperature of the solvent medium. The selection of pressure conditions is not critical to the operation of the process of the present invention within normal ranges of pressures generally employed in the laboratory.

The leaving group defined as A encompasses for example leaving groups normally employed in the chemical art such as a halogen, e.g., chloro, bromo or iodo substituents, the mesyloxy and tosyloxy groups, etc. In a preferred embodiment, leaving group A is preferably selected from the enumerated halogens and most preferably is chloro.

III

wherein R R and R are as above, via an intermediate compound of the following formula wherein R R and R are as above.

Compounds of Formula III are converted into compounds of Formula IV most conveniently by reacting the former with ethylene oxide in the presence of an aprotic Lewis acid such as a Friedel-Crafts catalyst, e.g., boron trifluoride, stannic chloride, titanium tetrachloride, and aluminum chloride. A most preferred catalytic material for the purposes of this reaction is aluminum chloride. Conventional solvents useful in Friedel-Crafts type reactions may be employed. A most preferred class of solvent include the aromatic hydrocarbons such as for example, benzene, xylene, toluene etc. The reaction may be conducted at a temperature in the range of from about -45 C., most preferably in the range of from about 30-40 C. However, even higher temperatures may be utilized providing that the system be placed under pressure so as to prevent loss of the volatile ethylene oxide.

It should be noted that when R; and/or R have the meaning amino in the final compound of Formula I above, it is preferred that such compounds be prepared by reduction of the corresponding R and/or R nitro groups in a manner known in the art subsequent to the ethylene oxide step. This is due to the fact that amino substituents in compounds of the Formula III would react with the ethylene oxide reagent. Thus, subsequent conversion of the nitro group to the amino group after the ethylene oxide step is indicated.

Transformation of compounds of Formula IV into corresponding compounds of Formula II will, of course, proceed in alternative fashion, depending upon the nature of leaving group A. In the case where leaving group A is one of the three enumerated halogens, a halogenating agent can be utilized in this reaction. Suitable halogenating agents include for the case of chlorine, thionyl chloride, phosphorus trichloride phosphorus pentachloride, etc. In the bromine embodiment, hydrogen bromide or phosphorus tribromide are suitable reagents Whereas hydrogen iodide is the reagent of preference for the iodine embodiment. The tosyl or mesyl halides form a preferred group of reagents useful for insertion of the tosyloxy or mesyloxy groups as members of leaving group A. In the reaction of compounds of Formula IV with the respective leaving group reagents, any solvent inert to the reactants may be utilized. Examples of such solvents include halogenated hydrocarbons such as methylene chloride, chloroform, etc. and the aromatic hydrocarbons such as benzene, toluene, xylene, etc. A temperature in the range of from 0 C. to 200 C. preferably from 0 C. to boiling point of the reaction medium is used. It is also possible 4 to utilize the leaving group reagent in excess as the solvent medium. In such instances a temperature in the range of from about 0 to 15 0 C. may be employed.

In another process embodiment of this invention compounds of Formula I may be prepared by the cyclization of compounds of the following formula N-CHZCHZA where R R R and A are as above. This cyclization reaction is conducted in the presence of a base such as a carbonate, e.g., potassium or sodium carbonate, or an alkali or alkaline earth hydroxide, etc. A useful solvent medium for this reaction comprises an aqueous lower alkanol, e.g., aqueous ethanol. Additionally, the reac tion medium may contain an organic base, such as pyridine or triethylarnine which serve as both solvents and added bases. The reaction conditions employed will be the same as previously described for the preparation of compounds of Formula I from compounds of Formula II.

The compounds of Formula V above may be conveniently prepared by first reacting hydroxylamine or an acid salt thereof, e.g., hydroxylamine hydrochloride with a compound of Formula IV so as to produce an oxime of the following formula where R R and R are as above; and then transforming the above oxime into the corresponding compounds of Formula V in the same manner previously described for the conversion of compounds of Formula IV into the compounds of Formula II, such as for example in a preferred embodiment where A is chloro, by the use of thionyl chloride at reflux in a halogenated solvent, e.g., methylene chloride.

Compounds of Formulae V and VI are novel intermediates and as such are considered to be a part of the present invention.

Compounds of the following formula VII wherein R R and R are as above, are known to be valuable therapeutic agents and are useful as muscle relaxants, sedatives and anti-convulsants.

Compounds of Formula I are readily converted into compounds of Formula VII by a reduction step whereby the oxygen atom on the 4-nitrogen is reductively eliminated.

The aforesaid reduction reaction may be carried out catalytically in the presence of hydrogen utilizing conventional hydrogenation catalysts such as Raney-nickel for this purpose. In a more preferable embodiment, especially When substituents R and/or R encompass groups sensitive to catalytic reduction, a chemical reducing agent such as phosphorus trichloride is employed. In the latter embodiment, a temperature in the range of from about C. to the reflux temperature of the reaction medium is utilized. Conventional inert solvents for this reduction step can be employed. Suitable solvents include, for example, the halogenated hydrocarbons such as methylene chloride, chloroform, etc. or the aromatic hydrocarbons such as, for example, benzene, toluene, Xylene, etc.

The following examples are illustrative but not limitative of the invention. Other embodiments will be obvious to those skilled in the art and are comprehended by the scope of the invention. All temperatures are stated in degrees centigrade, and all melting points are corrected.

EXAMPLE 1 Conversion of chloro 2 (2-chloroethylmethylamino) benzophenone into 7 chloro 2,3-dihydro-1-rnethyl-5- phenyl-lH-l,4-benzodiazepine 4-oxide To a solution of 5-chloro-2-(2-chloroethylmethylamino)benzophenone (2 gm., 6.5 mM.) in ethanol ml.) was added a solution of hydroxylamine hydrochloride (4.52 gm., 65 mM.) and potassium carbonate (4.14 gm., 30 mM.) in water (10 ml.). The mixture was stirred and refluxed for 24 hours, and was then concentrated in vacuo, and diluted with water. The mixture was made basic with sodium hydroxide solution, and the crude product was recovered by extraction with methylene chloride. Evaporation of the extract gave a yellow foam (1.82 gm.), which was dissolved in benzene, and purified by filtration through a column of Woelm activity III neutral alumina (18 gm.). Evaporation of the benzene eluate afforded nearly pure product as a yellow partly crystalline gum. This material became completely crystalline when triturated with ether or ether-hexane, giving the above-titled product as pale yellow prisms, M.P. 165167 C.

EXAMPLE 2 Preparation of 5-chloro-2-(2-hydroxyethylmethylamino) benzophenone To a stirred suspension of anhydrous aluminum chloride (79.8 gm., 0.6 mol) in dry benzene (200 ml.) was added a solution of 2-methylamino-5-chlorobenzophenone (73.7 gm., 0.3 mol.) in dry benzene (200 ml.) during 1.5 hours. The temperature rose slowly to 40 C. The mixture was allowed to cool spontaneously to 3035 C., and a solution of ethylene oxide (44.0 gm., 1.0 mols.) in dry benzene (100 ml.) was added dropwise during 1.5 hours, maintaining the temperature at 30-35 C. by using a water-bath. The progress of the reaction was followed by thin layer chromatography (TLC) of samples on plates of silica gel =F254 (Merck), which were developed with ethyl acetate, and examined under short wave UV light. To complete the conversion two further portions of ethylene oxide were added as previously, with periods of several hours in between additions. The reaction mixture was then poured onto ice (1.5 kg.), followed by addition of concentrated hydrochloric acid (300 ml.). The aqueous acid layer was separated, and the organic layer was further extracted with 3 N-hydrochloric acid (5X 200 ml.). The combined aqueous extracts were cooled, made basic with 40% (w./v.) sodium hydroxide solution, and extracted with methylene chloride. The extract was Washed with saturated aqueous sodium chloride, and dried over sodium sulfate. Evaporation aflords the crude product as a red oil, which was dissolved in benzene and filtered through a column of Woelm activity I neutral alumina (850 gm.). The benzene eluate was discarded; the purified product was obtained by elution of the column with methylene chloride, and evaporation of the eluate, to give 5-chloro-2- (2-hydroxyethylmethylamino) benzophenone as a yellow oil which was identical with an authentic sample by comparison of TLC and IR data.

EXAMPLE 3 Preparation of S-chloro-Z-(2-chloroethylmethylamino) benzophenone A solution of 5 chloro 2 (Z-hydroxyethylmethylamino) benzophenone (23.1 gm., 80.3 mM.) and pyridine (2 drops) in methylene chloride (200 ml.) was stirred and treated dropwise with a solution of thionyl chloride (14.4 ml., 23.8 gm., 200 mM.) in methylene chloride ml.) during 1.5 hours, followed by refluxing for 2 hours, with protection from atmospheric moisture. The mixture was evaporated in vacuo and excess thionyl chloride removed by co-distillation with methylene chloride. Ice-water (200 ml.) and 3 N-sodium hydroxide solution ml.) were added, and the crude product was recovered by extraction with methylene chloride. The extract was washed with saturated aqueous sodium chloride, dried over sodium sulfate, and evaporated in vacuo to give product as a dark red oil. This was dissolved in benzene, and purified by filtration through a column of Woelm activity I neutral alumina (230 gm.). Evaporation of the "benzene eluate gave pure product as a yellow oil identical with an authentic sample by comparison of TLC and IR data.

EXAMPLE 4 Deoxygenation of 7-chloro 2,3 dihydro 1 methyl-5- phenyl 1H 1,4 benzodiazepine 4-oxide to 7-chloro- 2,3-dihydro-l-methyl-S-phenyl-1H1,4benzodiazepine A solution of 7 chloro 2,3 dihydro 1 methyl-5- phenyl 1H 1,4 ibenzodiazepine 4-oxide (1 gm., 3.48 mM.) in methylene chloride (40 ml.) was treated with phosphorus trichloride (1 ml., 1.57 gm. 11.5 mM.), and refluxed for 0.5 hr., with protection from atmospheric moisture. The cooled reaction mixture was poured onto ice, and was then made basic with sodium hydroxide. Evaporation of the washed extracts gave V as a light brown gum which was converted into the monohydrochloride by treatment with excess methanolic hydrogen chloride, and precipitation with ether. The nearly pure 7 chloro 2,3 dihydro 1 methyl 5 phenyl-lH- 1,4-benzodiazepine hydrochloride was thus obtained as orange prisms, M.P. 248250 C. (sealed tube).

EXAMPLE 5 Preparation of 2-[2-benzoyl-4-chloroN-methylanilino]ethanol oxime A solution of 5.9 g. (20.4 mmols) of 5-chloro-2-(2- hydroxyethylmethylamino)benzophenone, 2.8 g. (41 mmols) of hydroxylamine hydrochloride and 45 ml. of pyridine was refluxed with protection from moisture for 22 hours. After cooling, most of the pyridine was removed in vacuo, the residue poured into water and extracted with methylene chloride. The methylene chloride extracts were washed with water, dried (MgSO and concentrated and allowed to stand at 0 to induce crystallization. The above-titled crude product was recrystallized from benzenezpentane to give 3.3 g. (53 percent) of white tan crystals, M.P. 109.5111.5 C. The analytical sample was prepared by recrystallization from the same solvent, M.P. 113-114 C.

7 EXAMPLE 6 Preparation of 7-chloro 2,3 dihydro 1 methyl-5- phenyl 1H 1,4 benzodiazepine 4-oxide from 2-(2 benzoyl-4-chloro-N-methylanilino) ethanol oxime A solution of 2-(2 benzoyl 4 chloro N methylanilino)ethanol oxime, (25 gm., 180.3 mM.) and pyridine (2 drops) in methylene chloride (200 ml.) was stirred and treated dropwise with a solution of thionyl chloride (14.4 ml., 23.8 -gm., 200 mM.) in methylene chloride during 1.5 hours, followed by refluxing for 2 hours with protection from atmospheric moisture. The moisture was evaporated in vacuo and excess thionyl chloride was removed -by co-distillation with methylene chloride. Icewater (200 ml.) and 3 N sodium hydroxide solution (100 ml.) were added and the crude 2-(2 benzoyl-4- chloro N methylanilin)ethyl chloride oxirne product was recovered by extraction with methylene chloride. The extract was washed with saturated aqueous sodium chloride, dried over sodium sulfate and evaporated in vacuo. The residue was dissolved in benzene and purified by filtration through a column of Woelm activity I neutral alumina (230 gin). Evaporation of the benzene gave the pure chloro-oxime.

To a solution of 2 (2 benzoyl 4 chloro-N-methylanilino)ethyl chloride oxime (2.14 gm., 6.5 mM.) in ethanol (20 ml.) was added a solution of potassium carbonate (4.14 gm., 30 mM.) in water (10 ml.). The mix ture was stirred and refluxed for 24 hours, and was then concentrated in vacuo, and diluted with water. The mixture was made basic with sodium hydroxide solution and the crude product was recovered by extraction with methylene chloride. The residue obtained after evaporation of the solvent was dissolved in benzene, and purified by filtration of Woelm activity III neutral alumina (18 gm.). Evaporation of the benzene eluate afforded a nearly pure product as a yellow crystalline gum. This material became completely crystalline when triturated with ether or ether-hexane, giving 7-chloro 2,3 dihydro-l-methylphenyl 1H 1,4 benzodiazepine 4-oxide as pale yellow prisms, M.P. 16Sl67 C.

We claim:

1. A process for the preparation of compounds of the formula wherein R and R each are selected from the group consisting of hydrogen, halogen, trifluoromethyl, nitro, amino, lower alkanoyl-amino, lower alkylthio, lower alkyl sulfonyl, lower alkylsulfinyl, lower alkoxy, hydroxy, lower alkyl, cyano, carboxy, carbo-lower alkoxy, carbamyl, and di-lower alkylamine; and R is selected from the group consisting of hydrogen, lower alkyl, lower alkenyl and 8 lower alkanoyl; said process comprising cyclizing a compound of the following formula /NCH2CHgA R2 OH wherein R R and R are as in claim 1, with hydroxylamine or an acid salt thereof so as to produce a compound of the following formula wherein R R and R are as above; and (b) reacting a compound of Formula VI above with a leaving group reagent to produce the desired compound of Formula V.

5. The process of claim 4 wherein said leaving group reagent is thionyl chloride.

References Cited Smith: Open-Chain Nitrogen Compounds, vol. 2 (W. A. Benjamin, Inc., N.Y., 1966), pp. 44-45.

ALTON D. ROLLINS, Primary Examiner US. Cl. X.R.

260-456 A, 465 E, 471 'R, 517, 558 A, 559 A, 562 P, 566 A, 570 A 

